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In spite of this, this plant species is over harvested and widely used in the culinary arts  and in traditional medicine as an infusion or powder for the treatment of digestive disorders.
Recently, analyses of essential oil of this species showed that is rich in α-terpinene (42.23%) and thymol (23.95%), which gives it a significant antifungal, antibacterial and antioxidant activity suggesting prospects for its application in food, cosmetics and pharmaceutical industry [13,14].
A high number of shoot (6.39 ± 0.21) was obtained on MS medium supplemented with 4.44 μM of 6-benzylaminopurine.
Regenerated shoots were rooted on plant growth regulators-free MS medium.
To investigate the effect of PGR on the proliferation and on the growth of axillary bud shoot, nodal segments (1 cm long) with a pair of axillary buds were placed on MS medium containing different concentrations of cytokinins, 6-benzylaminopurine (BAP - 2.2, 4.4, 6,6 and 8.8 μM) or Kinetin (KIN - 1.8, 4.6, 6.9 and 9.3 μM) combined or not with two concentrations of auxin, α-naphthalene acetic acid (NAA - 0.5 or 1 μM) Table 1.
After 3 weeks of culture period, data were evaluated as regeneration rate, number and length of micropropagated shoots.-rooted shoots were removed and washed to remove the agar from the roots.
Initially, nodal explants collected from wild plant were used for in vitro culture establishment on Murashige and Skoog (MS) basal salts medium without plant growth regulators (PGR).
Axillary shoot bud proliferation was achieved using plant growth regulators, 6-benzylaminopurine, and kinetin and α-naphthalene acetic acid.
Citation: Aicha N, Abdelmalek ELM (2014) Rapid in Vitro Regeneration and Clonal Multiplication of Thymus Bleicherianus Pomel, a Rare and Threatened Medicinal and Aromatic Plant in Morocco. doi:10.4172/2167-0412.1000145Copyright: © 2014 Aicha N, et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The wild plants were transferred and maintained in large pots, then they were transplanted in the garden of National Institute of Medicinal and Aromatic Plants; Taounate.
University 1of Sidi Mohamed Ben Abdellah– Fez–Morocco.
Shoot segments of about 5-6 cm long were harvested from mother plants and were then washed thoroughly under running tap water for about 30- 60 min.